Isolation of Clostridium Tetani from Soil.

Common mesophilic anaerobes, including Clostridium botulinum and Clostridium tetani, in 21 soil specimens.

A relatively rich medium was markedly superior to a dilute medium for the isolation of anaerobic bacteria from soil. The obligate anaerobes isolated from 21 soil samples were all clostridia and the counts ranged from 2.7 X 10-2 to 3.3 X 10-6 per g. The organisms most frequently isolated were Clostridium subterminate, C. sordelii, C. sporogenes, C. indolis, C. bifermentans, C. mangenoti, and C. ...

The Incidence of Clostridium Tetani in the Soil of Calcutta

Sixty samples of soil collected from different areas were examined for the presence of Clostridium tetani. The samples were collected during the pre-monsoon hot weather and the methods employed in the isolation and study of the cultures we're on the lines found satisfactory by Fildes (1925a and b). A summary of the methods employed is outlined below :? Approximately 2 to 3 c.cm. of ground-up so...

Isolation of Toxigenic Strains of Clostridium perfringens from the Soil of Farms in Iran

Comparative Study of Three Vaccinal Strains of Clostridium tetani Including Harvard 52, G5 and 49205 from Standpoint of Six Essential Factors to Evaluate Their Toxigenesis for Use in Tetanus Vaccine Production

Tetanus is an important disease which created by tetanospasmin toxin of Clostridium tetani. In this study we surveyed six important factors including LF, KF, MLD, pH, OD and total protein assay of Harvard 52 (H52), G5 (HG5) and 49205 (H49205) strains of the bacterium to determine which of them were more suitable for use in vaccine production. The mentioned strains were seperatedly reactivated i...

The Isolation of Atoxic Strains of Bacillus Tetani in Egypt

While investigating the Bacillus welchi content of the stools of Egyptians we observed that a very large number of samples of faeces grown in Robertson's bullock heart medium produced terminal drumstick spore-bearing bacilli morphologically resembling Clostridium tetani. Accurate figures concerning the incidence of clinical tetanus in Egypt are not available. For the past decade in Kasr el Ainy...